About

My name is Gal Haimovich.

I am a Postdoc at a leading laboratory that uses advanced fluorescent microscopy techniques to study regulation of gene expression. Since these techniques were new to me (practicaly, if not theoretically), I decided to use this blog as a platform to study this field. See also my opening post. [Note – several years later, I am still learning. This is a very exciting field!]

A list of my publications can be found here.

Professional links: ORCID   LinkdIn ResearchGate

As of 5/2016, I am an associae editor of the e-journal Bio-Protocol.

I am married to a beautiful woman and have two amazing kids, a boy and a girl. Both kids, I’m proud to say, are interested in science. My wife is interested in working with yeast as well, but only for baking.

keshet peleg miri peptides gal large

Our names, written as peptide & sugar structures…

 

Note: English is not my native tongue, so please excuse grammar and spelling errors.

If you can help a poor postdoc, I’d really appreciate it.
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16 responses to “About

  1. תסלח לי שאני מגיבה בעברית, נכון?
    אבל מזלטוב על הבלוג! יאי!

    Like

  2. Pablo Aguilar

    Hi Gal, thanks a lot for your post, very instructive. I am wondering if you have a plasmid to express LSSmKate2 in S. cerevisiae. I need to distinguish 3 colors by FACS and have only 488 and 633 excitation lines. Certainly LSSmKate2 would be very helpful. Thanks!

    Like

    • Hi Pablo,
      I am actually in the process of constructing one for myself, but as a fusion protein. I still don’t know how well it works in yeast. Once I get it working, I will report here.
      Anyway, you can get a plasmid with this protein at Addgene, or directly from the Verkhusha lab http://www.einstein.yu.edu/faculty/profile.asp?id=10316 (they actually developed this protein). I got it directly from them, and they were really nice and helpful.
      Hope that helps.

      Like

  3. Pablo Aguilar

    Thanks Gal!

    Like

  4. Hello Gal! I signed up to follow your blog a while ago and now I’m on WP more so hopefully I’ll catch up with what you’re saying.

    Like

  5. Hello Gal,
    Thank you for posting such a wonderful information on you blog. Very helpful ! I was wondering whether someone is using multiple mcherry tag (like 3x mcherry or 5x mcherry) in their study. I could not find it, if you have any information?

    Thanks,
    Shirish

    Like

  6. מזל טוב!
    שמחה לראות שהצטרפת לשורותינו.

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  7. Hi Gal,

    I was just finishing up reading a few of your more recent posts on Green Fluorescent Blog. I really like the content that you come up with. I wanted to further reach out to you because I have a team of writers and we would love to contribute to your site by creating premium editorial posts – which all feature fresh, trending content developed by our in-house team, tailored to your audience.

    You reap the benefits with unique, compelling posts aimed at your target audience to improve your website visibility, drive traffic and promote social interest & shares. All posts we create are original and developed for your site only.

    If you already have an editorial calendar in place, we’d like to contribute! Or, if you’re new to these opportunities, we encourage you to give it a try.
    Please do let me know if this caters to your interest.

    Looking forward to your positive response!
    Isabel Rocca

    Like

    • Hi Isabel,
      Thanks for your interest, but I’d rather choose and write my own contents. As I mentioned in my opening post, this blog is intended as a way for me to better understand this field. Having to do the reading, research, experience and writing helps me. Having other people do that for me will not help me.

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  8. Hi Gal,
    I stumbled upon your blog while looking for some information about MS2-MCP fusions. I work with Drosophila and I have engineered 6 MS2 loops in Drosophila gene that codes for non-coding RNA and now trying to visualize these ncRNAs with the Hsp83-MCP-GFP fusion. (This non-coding RNA binds to X-chromosome, so, I will be looking for (X-chromosome)-(ncRNA-MS2)- (MCP-GFP) interaction) but I haven’t had luck with the visualization yet. I am using either epiflourescence / confocal microscopy for visualization.I always get blazing green nucleus instead of specific MCP-MS2 interactions in the form of a focused spots/specks when we use entire tissue samples. abcam/ invitrogen GFP antibody did not work for me for immunostaining. I understand that since hsp83 is a constitutive promoter, i might be having lot of free unbound MCP-GFP. That might explain the entire green nucleus but apart from that, what could be the potential issue?.. I am fixing the drosophila embryos or tissue in formaldehyde based fixative. methanol kills GFP so cannot use alcohol/acetone based fixation. Do you happen to know good antibody against MCP protein itself which I could use instead of anti-GFP antibody. (I know it sounds counter intuitive but I am now hungry for this visualization result) Do you have any suggestions for me?
    Thanks again.
    Manasi

    Like

    • Hi Manasi,
      I’m currently on vacation so I’ll be brief

      Does ur mcp constract has an NLS?
      This will cause hi bckground in nucleus

      Try without the nls

      Are u sure the RNA-MBS is expressed? I would try FISH against the MBS. Use 50mer porobe. It should reduce background

      Like

  9. Hi ,my blog http://cmos360.wordpress.com/ ,We can add another link! We are the excellent microscope camera maker.

    Best regards!

    Like

  10. Hi Gal,
    My name is Laura Roberts. I am a graduate student at University of Wisconsin-Milwaukee, and I am conducting a study about PubPeer for a course I am taking on qualitative research. The purpose of my study is to research how and why scientists use PubPeer, and specifically what motivates them to comment on others’ findings anonymously.

    I came across your blog while researching PubPeer, and I am wondering if you would be interested in being a participant in my research study. As a participant, you would complete an online survey, answer interview questions and/or maintain a log of your commenting activities. The online survey consists of 17 questions and should take approximately 5 minutes to complete. The interview will consist of my asking about your commenting habits and opinions on using the website and will take up to 30 minutes. For the logs, I will ask you to record when you comment on the website and the nature of your comments over the course of a week.

    Could you email me at rober424@uwm.edu if you are interested in participating in my study?

    Thank you for your time and consideration,
    Laura Roberts

    Like

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