Single molecule FISH is currently the best method to get accurate measurements of mRNA levels at single molecule, single cell level in cell culture or tissue slices with a spatial resolution of ~200 nanometer (or less). One of the drawbacks of this method is the deterioration of the fluorescent signal (bleaching) of the organic dyes that are used to label the probes. Andrew Smith’s lab from University of Illinois now show how FISH can work with quantum dots instead of organic dyes. This provides better fluorophore stability and also the possibility to have more colors with less overlap of the emission spectra.
Most recent posts:
Posts are categorized as follows:
Tags3D-SIM ASAPbio ascb15 Bacteria BFP Bio-protocol BioimageXD bioRxiv blinking Brain brightness CASFISH CellProfiler CFP Channelrhodopsin Chicken Chrimson Chronos circularly permutated CLARITY clover CRISPR CSHL cy3 CyTOF DAPI definitions DiD DiL DiO DNA FISH dTomato E. coli EC EL222 emerald EoS ER ER-tracker ERK exosomes EYFP Fiji FISEB Fish (the animal) FISH-Quant FISSEQ FITC FlAsH flu Fly FM dyes folding FUNCAT gaussian fit GCaMP GFP GFPm GFPmut2 GFPmut3 glutamate sensor glycoRNA GRC HaloTag HHMI Janelia histones HIV(AIDS) Hoecht Icy ImageJ imaging_at_home ISCR Its broken! IVF Kinesin LanYFP laser localization LSSmKate LSSmOrange Lysotracker Malaria Mammalian cell maturation mCherry mCLING membrane nanotubes microvesicles mitochondria MitoTracker mKate2 mNeonGreen mRNA decay mRNA export mRNA localization MS2 mutations my pics nanobodies Nat Methods neuraminidase neurons NIH Image Nobel prize not imaging NPC oligomerization omnidirectional oocyte PALM PAmCherry peer-review peptide peroxisomes personal experience pHluorin photoinhibition photostability phototoxicity pHTomato Pol II CTD PP7 probe design propidiume iodide proteome QCBNet Qdot625 Qdot655 quantitative microscopy QY RCas9 ReaChr receptor internalization resolution RFP Rhodamine 123 ribosomal proteins RNA-Mango RNA2019 RNAScope Rodent science career shutter SiNC Singer lab single cell RNA-seq single molecule SmartFlare spaghetti monster spectrum SPIM Spinach split-GFP spot analysis STORM stress granules Suntag super-resolution supercharger superfast superfolder superglo TagRFP657 tenure track tethering tetramethylrosamine tFT TGF-beta transcription translation TRICK tRNA UnaG venus Worm yeast zebra fish zygote
Archive of Green Fluorescent Blog
Help me attend a conference