How to…

Calculate pixel size on images

Use FISH-Quant

Choose the right fluorescent proteinย (part 1) (part 2) (part 3)

Calculate numerical aperture & resolution

FISH for RNA (basics) (basics of spot analysis) (RNAScope)





3 responses to “How to…

  1. Hey!
    I wondered if it was somehow possible to do fluorescence microscopy with a microscope not made for the specific thing.


    • No, not really. A fluorescent microscope needs, at the very minimum, to have a light source suitable for fluorescent microscopy and the filter cube that houses the dichroic mirror(s) and filters. All these need to be properly assembled so the light will go through a certain path accurately. If the microscope frame is not built to house these, then you just canโ€™t do that.
      Moreover, there are objective lenses which are suitable for fluorescent microscopy, and those which are not.
      Tere are simple fluorescent microscopes that are relatively cheap.


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